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2 months ago | 
veterinaryresearch.biomedcentral.com | Agri-Product Safety
The BHK-21 cell clone BSR T7/5 stably expressing the phage T7 RNA polymerase was gifted from Prof. Zhigao Bu (Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China). The chicken embryonic fibroblasts (CEF) were grown and maintained in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% foetal bovine serum, respectively, at 37 °C with 5% CO2 atmosphere.
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Jan 15, 2025 | 
veterinaryresearch.biomedcentral.com | Agri-Product Safety
To explore and investigate the relationship between TRIM8 and PEDV infection, we measured the expression of TRIM8 in PEDV-infected IPEC-J2 cells using qRT-PCR and western blot analysis. The results showed that the expression level of the TRIM8 gene was significantly upregulated at 12, 24, and 48 h of infection, with the highest level observed at 24 h post-infection (Figure 1A).
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Jan 11, 2025 | 
veterinaryresearch.biomedcentral.com | Agri-Product Safety
Research on virus-host interactions has long been a focus of study. This field not only uncovers the mysterious roles of viral proteins and the complex mechanisms behind viral pathogenicity [93,94,95] but also lays the groundwork for developing new antiviral drugs [96,97,98]. NDV, an essential representative of paramyxoviruses, has provided valuable insights into the unexplored replication and pathogenesis of paramyxoviruses [99,100,101].
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Jan 9, 2025 | 
onlinelibrary.wiley.com | Yuxi Feng |Zhao Chen |Agri-Product Safety |Luyun Chen
Supporting Information Filename Description ppl70044-sup-0001-Supinfo.pdfPDF document, 980.3 KB Data S1: Supporting Information REFERENCES , & (2020). Genetic diversity of salt tolerance in tetraploid alfalfa (Medicago sativa L.). Acta Physiologiae Plantarum, 42, 5. & (1985). Breeding crop varieties for stress environments. Critical Reviews in Plant Sciences, 2, 199–238. , & (2022).
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Oct 20, 2024 | 
academic.oup.com | Agri-Product Safety
Uterine fluid homeostasis during peri-implantation is crucial for successful embryo implantation. Taurine (Tau) plays a crucial role in regulating osmotic pressure and ion transport. However, the precise mechanisms underlying Tau-mediated regulation of uterine fluid homeostasis during peri-implantation in mice remain unclear.
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Oct 2, 2024 | 
genomebiology.biomedcentral.com | Agri-Product Safety
The GWAS panel consisted of 299 cucumber accessions, which were acquired as seeds from the USA national plant germplasm system collection, the Yangzhou University cucumber breeding lab, or from commercial sources (Additional file 1: Table S1). Collection and use of plant materials was conducted in accordance with institutional, national, and international guidelines and legislation.
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Sep 28, 2024 | 
academic.oup.com | Agri-Product Safety
Increasing agricultural losses caused by insect infestations are a significant problem, so it is important to generate pest-resistant crop varieties to address this issue. Several reviews have examined aphid–plant interactions from an entomological perspective. However, few have specifically focused on plant resistance mechanisms to aphids and their applications in breeding for aphid resistance.
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Aug 27, 2024 | 
academic.oup.com | Agri-Product Safety
Chickens are the most thoroughly domesticated vertebrate species, and after long-continued natural and artificial selection, they now show rich phenotypic diversity. In particular, feathered legs present in domestic chickens are a characteristic that is carefully selected by advanced breeders. Previous studies have identified the key mutations responsible for feathered legs on chromosomes 13 and 15; however, not all chickens can be easily distinguished based on these two markers.
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Aug 27, 2024 | 
academic.oup.com | Agri-Product Safety
As an important post-translational modification, ubiquitination plays an important role in regulating protein homeostasis in eukaryotic cells. In our previous studies, both the transcriptome and proteome suggested that ubiquitination is involved in the formation of chicken primordial germ cells (PGCs).
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Aug 23, 2024 | 
bmcgenomics.biomedcentral.com | Agri-Product Safety
The whole genome and protein sequences of R2R3-MYB transcription factor subfamily genes in rice (Oryza sativa L.) were obtained from the Rice Genome Annotation Project (http://rice.uga.edu/index.shtml) [57], Rice Annotation Project Database (RAP-DB, https://rapdb.dna.affrc.go.jp/index.html) [58], and Plant Transcription Factor Database (http://planttfdb.gao-lab.org/) [59].
