Aher Jayesh Bhausaheb

Sep 8, 2024 | biorxiv.org | Aher Jayesh Bhausaheb |Debraj Koiri |Sandeep Choubey |Jafarulla Shaikh

AbstractPathogenic bacteria evade host defense by rupturing the phagosomal compartment, enabling their escape into the host cytosol. The bacteria is known to establish direct physical contact with the host compartment prior to phagosome disruption. However, the impact of direct-physical contact on the phagosome remodeling and deformation remains elusive.

Apr 25, 2024 | biorxiv.org | Debraj Koiri |Mintu Nandi |Abik Hameem PM |Aher Jayesh Bhausaheb

AbstractMycobacterium tuberculosis (Mtb) evades host defense by hijacking and rupturing the phagosome, enabling it to escape to the host cytosol for its survival. ESAT-6, a secreted virulence protein of Mtb, is known to be critical for phagosome rupture. However, the mechanism of ESAT-6-mediated disruption of the phagosomal membrane remains unknown. Using in vitro reconstitution and numerical simulations, we discover that ESAT-6 polymerization remodels and vesiculates phagosomal membrane.