Ajit R Ramamohan

Apr 21, 2024 | biorxiv.org | Elizabeth McFadden |Stephanie R. Monticelli |Albert Wang |Ajit R Ramamohan

AbstractCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus that can cause severe disease in humans with case fatality rates of 10-40%. Although structures of CCHFV glycoproteins GP38 and Gc have provided insights into viral entry and defined epitopes of neutralizing and protective antibodies, the structure of glycoprotein Gn and its interactions with GP38 and Gc have remained elusive.

Feb 10, 2024 | biorxiv.org | Patrick Byrne |Madeline R. Sponholtz |Alison Lee |Ajit R Ramamohan

AbstractHuman cytomegalovirus (HCMV) glycoprotein B (gB) is a class III membrane fusion protein required for viral entry. HCMV vaccine candidates containing gB have demonstrated moderate clinical efficacy, but no HCMV vaccine has been FDA-approved. Here, we used structure-based design to identify and characterize amino acid substitutions that stabilize gB in its metastable prefusion conformation.