Alyssa Semmelman

Nov 19, 2024 | biorxiv.org | Ziyuan Jiang |Deepti Karandur |Alyssa Semmelman |Neel Shah

AbstractMulti-domain signaling enzymes are often regulated through extensive inter-domain interactions, and disruption of inter-domain interfaces by mutations can lead to aberrant signaling and diseases. For example, the tyrosine phosphatase SHP2 contains two phosphotyrosine recognition domains that auto-inhibit its catalytic domain.

May 13, 2024 | biorxiv.org | Ziyuan Jiang |Deepti Karandur |Neel Shah |Alyssa Semmelman

AbstractMulti-domain enzymes can be regulated both by inter-domain interactions and structural features intrinsic to the catalytic domain. The tyrosine phosphatase SHP2 is a quintessential example of a multi-domain protein that is regulated by inter-domain interactions. This enzyme has a protein tyrosine phosphatase (PTP) domain and two phosphotyrosine-recognition domains (N-SH2 and C-SH2) that regulate phosphatase activity through autoinhibitory interactions.