Ananth Pallaseni

Feb 16, 2023 | nature.com | Jonas Koeppel |Juliane Weller |Ananth Pallaseni |Fabio Giuseppe Liberante

AbstractMost short sequences can be precisely written into a selected genomic target using prime editing; however, it remains unclear what factors govern insertion. We design a library of 3,604 sequences of various lengths and measure the frequency of their insertion into four genomic sites in three human cell lines, using different prime editor systems in varying DNA repair contexts.