Anastasia Treshchalina

1 month ago | mdpi.com | Elizaveta Boravleva |Anastasia Treshchalina |Daria Gordeeva |Alexandra Gambaryan

2. Materials and Methods 2.1. VirusesThe NDV/duck/Moscow/3639/2008 (d3639) virus was isolated from the feces of wild ducks sampled at the side of a pond in Moscow in 2008 (10 August 2008). The pathogenic NDV/chicken/Moscow/6081/2022 (ch6081) virus was isolated (31 August 2022) from a chicken, as described earlier [19]. The viruses were propagated in the allantoic cavity of pathogen-free, 10-day-old embryonated chicken eggs (ECEs) at 36 °C and harvested at 48 h post infection (p.i.).