Andreas K. Nüssler

Nov 2, 2023 | mdpi.com | Ritu Gupta |Sneha Gupta |Purva Gupta |Andreas K. Nüssler

3. Results and Discussion 3.1. Callus Formation and Growth CurveCQ leaf explants were inoculated with MS media enriched with suitable plant hormonal concentrations, resulting in successful induction of compact cream-coloured calli within 3 weeks, followed by brown-coloured friable calli formation in 6 weeks. The induced callus was subcultured at regular intervals of 30 days, by providing the same medium components and optimum growth conditions (Figure 1a).