Antonina Roll-Mecak

Dec 4, 2024 | onlinelibrary.wiley.com | Jeffrey O. Spector |Jiayi Chen |Ewa Szczesna |Antonina Roll-Mecak

Supporting Information Filename Description jmi13375-sup-0001-MovieS1.avi90.6 MB Movie S1: Movie showing the IRM channel (left) and GFP-EB1 channel (right) acquired simultaneously. The IRM channel was background subtracted and processed by a bandpass FFT. The movie was recorded at 10 frames/s and played back at 30 fps. Individual polymerisation and depolymerisation events are visible in the IRM channel. The GFP channel displays the EB1 comets. REFERENCES 1, & (1986).

Aug 28, 2024 | biorxiv.org | Jeffrey O. Spector |Jiayi Chen |Antonina Roll-Mecak

AbstractInterference Reflection Microscopy (IRM) is an optical technique that relies on the interference between the reflected light from an incident beam as it passes through materials of different refractive indices. This technique has been successfully used to image microtubules, biologically important biofilaments with a diameter of 25 nm. However, it is often desirable to image both the microtubule and microtubule interacting proteins simultaneously.