Brian Fulton-Howard

Nov 26, 2024 | cell.com | Kathryn R. Bowles |Chiara Pedicone |Derian A Pugh |Laura-Maria Oja |Brian Fulton-Howard |Sarah Weitzman | +5 more

CRISPR/Cas9 genome editingHuman iPSCs were edited using the pSpCas9(BB)-2A-GFP (PX458) construct backbone (Addgene #48138) described in Ran et al 201382. For WT correction of S305 mutation lines, specific guide RNAs (gRNAs) were designed against the mutant allele using the Zhang lab design tool (crispr.mit.edu), and guides with the highest quality scores and more than 3bp mismatches with other genomic loci were selected.