Bruce Curtis

Oct 31, 2024 | biorxiv.org | Morgan J. Colp |Cédric Blais |Bruce Curtis |John Archibald

AbstractIn this study, artificial transformation experiments were performed to investigate how the A. castellanii genome responds to foreign DNA presented in both circular and linear plasmid form. Nanopore sequencing was used as a high throughput method to screen for transgene DNA in the resulting transformant cultures, and candidate transgene integrations were identified.