Calina Glynn

3 weeks ago | nature.com | Sven Klumpe |Calina Glynn |Judy S. Kim |Michele C. Darrow |Michael Grange

AbstractScanning electron microscopy (SEM) of frozen-hydrated biological samples allows imaging of subcellular structures at the mesoscale in a representation of their native state. Combined with focused ion beam milling (FIB), serial FIB/SEM can be used to build a 3-dimensional model of cells and tissues. The correlation of specific regions of interest with cryo-electron microscopy (cryoEM) can additionally enable subsequent high-resolution analysis.