Calvin Lin

Aug 2, 2024 | biorxiv.org | Calvin Lin |Harry Li |Daniel Brogan |Tianqi Wang

AbstractType III-E CRISPR-Cas effectors, of which Cas7-11 is the first, are single proteins that cleave target RNAs without nonspecific collateral cleavage, opening new possibilities for RNA editing. Biochemical experiments combined with amide hydrogen-deuterium exchange (HDX-MS) experiments provide a first glimpse of the conformational dynamics of apo Cas7-11.