Carrie L. Partch

May 14, 2024 | nature.com | Emma Carroll |Carrie L. Partch |David J. Vocadlo

AbstractDifferential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by fluorogenic dyes. However, DSF applications remain limited by protein incompatibilities with existing DSF dyes. Here we overcome this obstacle with the development of a protein-adaptive DSF platform (paDSF) that combines a dye library ‘Aurora’ with a streamlined procedure to identify protein–dye pairs on demand.