Carrie L. Partch

Feb 13, 2025 | cell.com | Rebecca K Spangler |Keya Jonnalagadda |Jordan Ward |Carrie L. Partch

Keywordscircadian rhythmsCaenorhabditis elegansdevelopmentchronobiologygenetic oscillatorTimekeeping in natureMolecular timing mechanisms are vital in orchestrating life processes, directing events from cellular functions to organismal behaviors to optimize fitness and fecundity. Biological timers are tightly regulated and often integrate external signals to align internals processes with the outside world.

May 14, 2024 | nature.com | Emma Carroll |Carrie L. Partch |David J. Vocadlo

AbstractDifferential scanning fluorimetry (DSF) is a technique that reports protein thermal stability via the selective recognition of unfolded states by fluorogenic dyes. However, DSF applications remain limited by protein incompatibilities with existing DSF dyes. Here we overcome this obstacle with the development of a protein-adaptive DSF platform (paDSF) that combines a dye library ‘Aurora’ with a streamlined procedure to identify protein–dye pairs on demand.