Changfu Cao

Dec 27, 2024 | virologyj.biomedcentral.com | Li Sun |Jianbo Yang |Jian Jin |Changfu Cao

IPEC-J2 cells were maintained in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) at 37 °C and 5% CO2. The PEDV CV777 strain was generously provided by China Agricultural University. The IPEC-J2 cells were infected with PEDV at a multiplicity of infection (MOI) of 1 and then cultured in DMEM containing 2 µg/mL trypsin at 37 °C and 5% CO2 for 1 h. After incubation, the cells were washed with phosphate-buffered saline (PBS) and then cultured in DMEM containing 2% FBS.