Chidambareswaren Mahadevan

Mar 18, 2024 | biorxiv.org | Maria Font Farre |Daniel Brown |Reka Toth |Chidambareswaren Mahadevan

AbstractEukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes.