Chris Plott

Jun 21, 2023 | nature.com | Katrien Devos |Chris Plott |Avinash Sreedasyam |Jane Grimwood |Jerry Jenkins |John Lovell | +4 more

Genome sequencingSeed from a single self-pollinated plant of E. coracana subsp. coracana accession KNE 796-S was planted in trays and grown under artificial lighting (14 h days) at 27 °C. Seedlings were harvested approximately one month postemergence. For DNA extractions59, frozen leaves were ground to a fine powder in liquid nitrogen in a precooled mortar and gently extracted in a CTAB buffer that included proteinase K, PVP-40 and β-mercaptoethanol for 1 h at 50 °C.