Clare Brown

Dec 18, 2024 | homeschoolof1.com | Clare Brown

Winter bingo printables bring a cozy touch of fun to snowy days and chilly afternoons. This simple and engaging game is a favorite in our home, whether we’re spending time as a family or hosting a seasonal get-together. With its festive design and easy setup, it’s the perfect activity to brighten up cold-weather moments. Discover how these free printable bingo cards can add joy to your winter gatherings!**This post may contain affiliate links.

Dec 16, 2024 | homeschoolof1.com | Clare Brown

Our January word search printable is a favorite in our home—it’s a fun way to keep kids learning and entertained all winter long. It’s packed with seasonal words that celebrate January traditions. Whether you’re homeschooling or need a quick classroom activity, it’s perfect for keeping kids busy. On a chilly day, it’s great for some quiet fun indoors. This free printable word search is one of our favorites and makes learning both easy and enjoyable for kids!**This post may contain affiliate links.

Oct 10, 2024 | harrowells.co.uk | Clare Brown

Our Resolve. Your Resolution. Our articles are intended for general information purposes only and are not a substitute for professional advice tailored to your specific circumstances. We are always very happy to discuss any plans, issues or concerns you may have and to clarify how we might be able to help. We cannot accept responsibility for any loss as a result of acts or omissions taken in respect of this article.

Oct 7, 2024 | nationalworld.com | Clare Brown

Watch more of our videos on ShotsTV.com and on Freeview 262 or Freely 565Visit Shots! nowThe county of Warwickshire, right at the very heart of England, is famous for its natural beauty. Much lesser known is the key role that Warwickshire played during World War II and the so-called Cold War period. The county of Warwickshire, right at the very heart of England, is famous for its natural beauty with countless villages hidden away in the countryside – each one a gem for the curious visitor.

Oct 3, 2024 | nature.com | Victoria T. Chu |Abigail Glascock |Deborah Donnell |Cole Grabow |Clare Brown |Christina Love | +9 more

Doxycycline post-exposure prophylaxis (doxy-PEP) reduces bacterial sexually transmitted infections among men who have sex with men and transgender women. Although poised for widespread clinical implementation, the impact of doxy-PEP on antimicrobial resistance remains a primary concern as its effects on the gut microbiome and resistome, or the antimicrobial resistance genes (ARGs) present in the gut microbiome, are unknown. To investigate these effects, we studied participants from the DoxyPEP trial, a randomized clinical trial comparing doxy-PEP use, a one-time doxycycline 200-mg dose taken after condomless sex (DP arm, n = 100), to standard of care (SOC arm, n = 50) among men who have sex with men and transgender women. From self-collected rectal swabs at enrollment (day-0) and after 6 months (month-6), we performed metagenomic DNA sequencing (DNA-seq) or metatranscriptomic RNA sequencing (RNA-seq). DNA-seq data were analyzable from 127 samples derived from 89 participants, and RNA-seq data were analyzable from 86 samples derived from 70 participants. We compared the bacterial microbiome and resistome between the two study arms and over time. The median number of doxycycline doses taken since enrollment by participants with DNA-seq data was zero (interquartile range (IQR): 0–7 doses) for the SOC arm and 42 (IQR: 27–64 doses) for the DP arm. Tetracycline ARGs were detected in all day-0 DNA-seq samples and in 85% of day-0 RNA-seq samples. The proportional mass of tetracycline ARGs in the resistome increased between day-0 and month-6 in DP participants from 46% to 51% in the metagenome (P = 2.3 × 10−2) and from 4% to 15% in the metatranscriptome (P = 4.5 × 10−6), but no statistically significant increases in other ARG classes were observed. Exposure to a higher number of doxycycline doses correlated with proportional enrichment of tetracycline ARGs in the metagenome (Spearman’s ρ = 0.23, P = 9.0 × 10−3) and metatranscriptome (Spearman’s ρ = 0.55, P = 3.7 × 10−8). Bacterial microbiome alpha diversity, beta diversity and total bacterial mass did not differ between day-0 and month-6 samples from DP participants when assessed by either DNA-seq or RNA-seq. In an abundance-based correlation analysis, we observed an increase over time in the strength of the correlation between tetracycline ARGs and specific bacterial taxa, including some common human pathogens. In sum, doxy-PEP use over a 6-month period was associated with an increase in the proportion of tetracycline ARGs comprising the gut resistome and an increase in the expression of tetracycline ARGs. At 6 months of doxy-PEP use, no residual differences were observed in alpha and beta diversity or taxonomic composition of the gut microbiome. As doxy-PEP is implemented as a public health strategy, further studies and population-level surveillance of doxycycline-resistant pathogens are needed to understand the implications of these findings. ClinicalTrials.gov registration number: NCT03980223 . In participants from a randomized controlled trial, doxy-PEP use over 6 months minimally affected the gut microbiome’s taxonomic composition but increased the abundance and active expression of tetracycline antibiotic resistance genes.