Cornelia Holler

Feb 12, 2024 | nature.com | Cornelia Holler |Richard Taylor |Leonhard Möckl

AbstractDelivery of very small amounts of reagents to the near-field of cells with micrometer spatial precision and millisecond time resolution is currently out of reach. Here we present μkiss as a micropipette-based scheme for brushing a layer of small molecules and nanoparticles onto the live cell membrane from a subfemtoliter confined volume of a perfusion flow. We characterize our system through both experiments and modeling, and find excellent agreement.