David Silva-Sanchez

Oct 11, 2024 | biorxiv.org | Miro Astore |Robert Blackwell |David Silva-Sanchez |Pilar Cossio

AbstractCryogenic electron microscopy (cryo-EM) has emerged as a powerful method for resolving the atomistic details of cellular components. In recent years, several computational methods have been developed to study the heterogeneity of molecules in single-particle cryo-EM. In this study, we analyzed a publicly available single-particle dataset of TRPV1 using five of these methods: 3D Flexible Refinement, 3D Variability Analysis, cryoDRGN, ManifoldEM, and Bayesian ensemble reweighting.