Emilia Grypioti

Dec 3, 2023 | nph.onlinelibrary.wiley.com | Hugues Richard |Bioinformatics Unit |Emilia Grypioti |Nikoleta Kryovrysanaki

Introduction RNA interference (RNAi) is a conserved mechanism of regulation of gene expression mediated by small RNAs (sRNAs; Farazi et al., 2008; Ghildiyal & Zamore, 2009). Intracellular double-stranded RNAs (ds-RNA) are cleaved by RNase III-like ribonuclease enzymes of the Dicer (DCR) family into small (c. 20–35 nt) ds-sRNA, which are subsequently incorporated into an ARGONAUTE (AGO)-containing complex known as the RNA-induced silencing complex (RISC).