Eva Nogales

Nov 1, 2024 | sciencedirect.com | Nicole Haloupek |Eva Nogales |Michael Lisby |Si-Jin Cheng

The folding, stability, and function of a protein are determined by its amino acid sequence.1 Accordingly, gene variants that affect the amino acid sequence can result in proteins with changed structural, biophysical, and functional properties.2 In recent years, tremendous progress has been made in our understanding and ability to predict the consequences of single amino acid substitutions (missense variants) on the structural stability and function of proteins.2,3,4,5,6,7 In comparison, the...

Sep 19, 2024 | cell.com | Los Alamos |Eva Nogales |Simon Poepsel

Keywordsepigeneticschromatincryo-EMgene regulationdevelopmenttrans-activationPRC2histone methyltransferaseIntroductionPolycomb repressive complex 2 (PRC2) is an essential chromatin regulator that harbors histone methyltransferase (HMTase) activity mediated by its catalytic subunit enhancer of zeste homolog 2 (EZH2).

May 30, 2024 | nature.com | Eva Nogales

Macromolecules are involved in myriads of interactions that regulate their cellular function. While years of structural biology progress was built by reducing this complexity, a molecular understanding of biological processes requires the characterization of ever larger and more dynamic molecular assemblies. Cryo-electron microscopy is rising to this challenge.

Sep 13, 2023 | onlinelibrary.wiley.com | Eva Nogales |Elizabeth Kellogg

Microtubules (MTs) are ubiquitous cytoskeletal elements of eukaryotic cells that play a myriad of roles, all of them involving a number of microtubule-associated proteins (MAPs) that recognize, bind, and often regulate MT activity. Polymers of repeating αβ-tubulin subunits, MTs are made of protofilaments that associate in parallel and a staggered manner to give rise to a pseudo-helical array of tubulin dimers.