Facultade de Medicina

Jul 25, 2024 | academic.oup.com | Unidade de Xenética |Facultade de Medicina |Santiago de Compostela |Universidade da Coruña

Most tools for normalizing NanoString gene expression data, apart from the default NanoString nCounter software, are R packages that focus on technical normalization and lack configurable parameters. However, content normalization is the most sensitive, experiment-specific, and relevant step to preprocess NanoString data. Currently this step requires the use of multiple tools and a deep understanding of data management by the researcher.