Henan Agricultural

2 months ago | bmcvetres.biomedcentral.com | Henan Agricultural

We took 1,680 g of A. annua, added 6 times its volume of solution (distilled water and 70% ethanol), soaked it for one week, and then filtered it with 6 layers of gauze. The filtrate was centrifuged at a rotating speed of 3,000 r/min for 10 min to collect the supernatant, which was concentrated in a boiling water bath to expand the water. The final constant volume concentration was 1 g/mL (1,680 mL).

Jan 23, 2025 | link.springer.com | Henan Agricultural

AbstractWith the in-depth and comprehensive research on probiotic Bacillus, it has become a hot topic in food science. However, the current status of research using bibliometric analysis to assess the application of probiotic Bacillus in food science has not been comprehensively reviewed. The Web of Science (WOS) database was used in this review’s bibliometric analysis to determine the hotspots for research as well as the extent of completed experiments.

Nov 30, 2024 | jasbsci.biomedcentral.com | Henan Agricultural

Day-old male Ross-308 male chickens were purchased from Vervaeke-Belavi (Tielt, Belgium), and subjected to the vaccination against Newcastle Disease and Infectious Bronchitis at 1 days of age, followed by the vaccination against Newcastle Disease at 18-day-old. They were reared in a climate-controlled facility, in which the initial temperature was set as 32 °C during 1–3 d and then gradually decreased to 22 °C by 21 d.

Nov 28, 2024 | bmcvetres.biomedcentral.com | Henan Agricultural

All the primers, quenched fluorescent DNA reporter (FAM-TTATT-BHQ1) and LFS test reporter (FAM-TTATT-Biotin) were obtained from Sangon Biotech (Shanghai, China). All the nucleotide (nt) sequences are listed in Additional File 1: Table S1. Recombinant Francisella novicida Cas12a (FnCas12a) protein was purchased from Tolo Biotech (Shanghai, China). HiScribe™ T7 High Yield RNA Synthesis Kits were purchased from New England Biolabs (Ipswich, MA, USA).

Nov 17, 2024 | bmcgenomics.biomedcentral.com | Henan Agricultural

In this study, Hy-Line Brown laying hens were used as experimental animals. The hens were obtained from the Animal Center of Henan Agricultural University. A total of 200 hens were reared in cages under identical environmental conditions. At 15, 20, 30 and 68 W, three healthy hens were randomly selected from each age group, totaling 12 hens. Under deep anesthesia, euthanasia was performed via intravenous injection of potassium chloride (1–2 mg/kg).