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Jan 23, 2025 | 
link.springer.com | Hubei Hongshan Laboratory
AbstractKiwifruit is an economically and nutritionally important horticultural fruit crop worldwide. The genomic data of several kiwifruit species have been released, providing an unprecedented opportunity for pan-genome analysis to comprehensively investigate the inter- and intra-species genetic diversity and facilitate utilization for kiwifruit breeding. Here, we generated a kiwifruit super pan-genome using 15 high-quality assemblies of eight Actinidia species.
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Jan 13, 2025 | 
bmcgenomics.biomedcentral.com | Huazhong Agricultural |Hubei Hongshan Laboratory
All experimental animals in this study were offspring of M. amblycephala “Huahai No. 1”, which were bred simultaneously at the Ezhou breeding facility of Huazhong Agricultural University and reared under identical conditions in the same pool. At four months, total length (TL), body length (BL) and body weight (BW) of the fish were measured.
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Dec 5, 2024 | 
link.springer.com | Huazhong Agricultural |Hubei Hongshan Laboratory
AbstractCreatine is a feed additive with physiological pleiotropic properties and also an energy homeostasis protector in vertebrates and is successfully used in terrestrial livestock and aquaculture. Here, two feeding trials were performed to investigate dietary creatine on endogenous creatine metabolism and physiological reprogramming in largemouth bass.
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Dec 5, 2024 | 
genomebiology.biomedcentral.com | Hubei Hongshan Laboratory |Huazhong Agricultural
Seven Cas13 orthologs were codon-optimized and synthesized by Kingsley Company. The pRGEB32-GhU6.7-NPTII vector was linearized using BstBI and XbaI. Subsequently, the full-length Cas13 was ligated into the linearized pRGEB32-GhU6.7-NPTII using the ClonExpress® II One Step Cloning Kit to obtain pRGEB32-GhU6.7-Cas13-NPTII. The tRNA-gRNA transcription unit has been used to effectively enhance crRNA transcription in the CRISPR/Cas system for plant genome editing [47, 75, 76].
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Dec 2, 2024 | 
genomebiology.biomedcentral.com | Hubei Hongshan Laboratory |Huazhong Agricultural
The winter wheat cultivar AK58 was used in this study. After being sterilized with 75% ethanol for 45 s, the seeds were soaked in water containing 1.6% H2O2 for 24 h at room temperature and pre-germinated on wet filter paper. The germinated seeds were transferred to soil and grown in a growth chamber with 16 h light and 8 h dark cycle at 22 °C for normal growth and at 4 °C for vernalization treatment. For V0 treatment, plants were grown at 22 °C for 14 days.
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Dec 1, 2024 | 
genomebiology.biomedcentral.com | Huazhong Agricultural |Hubei Hongshan Laboratory
To determine whether a region of the genome qualifies as a TAD, we defined four representative TAD features: horizontal border strength (H), vertical border strength (V), vertex area (Va), and diamond score (Fig. 1). These features are extracted from the observed/expected matrix, computed using the Hi-C matrix normalized by iterative correction and eigenvector decomposition (ICE) [44].
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Oct 16, 2024 | 
bmcgenomics.biomedcentral.com | Hubei Hongshan Laboratory |Huazhong Agricultural
The experimental fish were sacrificed by anesthetized with MS-222 at 100 mg/L before fin collection and dissection. Then we performed a dissection of the gonads while the fish were still anesthetized to determine their sex. In the processes of sex-specific molecular marker screening, a total of 257 M. amblycephala (116 females and 141 males), nine populations from Yuni Lake (YNL, n = 30), Poyang Lake (PYL, n = 29), Liangzi Lake (LZL, n = 24), Hubei Zhenglong Aquatic Seed Industry Co., Ltd.
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Oct 14, 2024 | 
veterinaryresearch.biomedcentral.com | Huazhong Agricultural |Hubei Hongshan Laboratory
One hundred and eighty one-day-old Cherry Valley ducks were purchased from the Wuhan Yongsheng Duck Farm (Hubei, China) and housed at the laboratory animal centre of Huazhong Agricultural University. Throughout the study period, 10 ducks were housed in a cage in a temperature-controlled environment. The ducks were exposed to light for 12 h each day and had access to unlimited antibiotic-free food and water.
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Oct 10, 2024 | 
genomebiology.biomedcentral.com | Hubei Hongshan Laboratory |Huazhong Agricultural
Wild-type (WT) cultivar MH63 (Oryza sativa L. ssp. indica cv. Minghui 63) or osbzip23 T-DNA mutant seeds were soaked for 3 days until germination. Seedings with similar growth states were then planted in 12 buckets (30 plants per bucket) with equal amounts of soil and water. The buckets were randomly placed in a growth chamber with an air temperature of 32 °C (day) or 29 °C (night), 12 h of light (constant light intensity 15,000 lx) per day, and a relative humidity of 80%.
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Sep 16, 2024 | 
academic.oup.com | Hubei Academy |Hubei Hongshan Laboratory
The rise in MDR MRSA poses an increasing public health threat. For example, MRSA carrying the MDR gene cfr shows resistance to linezolid, which is often considered the last-line antimicrobial agent for the treatment of serious infections caused by resistant Gram-positive bacteria in humans.1 The staphylococcal cassette chromosome mec (SCCmec) is the main mobile genetic element responsible for the spread of mecA, but also acts as a carrier of other resistance genes.
