Jennifer Wright

Feb 20, 2023 | physoc.onlinelibrary.wiley.com | Clare M. Eglin |Jennifer Wright |Anthony I Shepherd |Heather Massey

2.1 Data analyses Data analyses were conducted on all samples. Samples that gave a negative expression value were considered as zero for analysis. The number of samples that lay 10% above or below the standard curve was noted, as was the number of samples that had a CV of >15% (Tables A1 and A2). Ideally, samples with a CV of >15% or which were >10% above the standard curve would have been removed from the analysis.

Feb 20, 2023 | physoc.onlinelibrary.wiley.com | Clare M. Eglin |Jennifer Wright |Matthew J. Maley |Sarah Hollis

2 METHODS The protocol received ethical approval from the Ministry of Defence Research Ethics Committee (909/MoDREC/18), and all participants gave informed written consent prior to any testing. The study complied with the Declaration of Helsinki (1964), as last revised at the 64th World Medical Association General Assembly, Brazil, 2013, except for registration in a database. Testing was undertaken between January 2019 and October 2019 when the ambient outdoor temperature was 9.5 (5.1)°C.

Feb 19, 2023 | physoc.onlinelibrary.wiley.com | Jennifer Wright

Feb 19, 2023 | physoc.onlinelibrary.wiley.com | Jennifer Wright

Feb 19, 2023 | physoc.onlinelibrary.wiley.com | Jennifer Wright |Heather Massey |Sarah Hollis |Tom Vale

2.3 Quantitative sensory testing The laboratory in which the QST protocol was conducted was quiet and maintained at 24.0 (0.8)°C. The QST protocol was conducted in accordance with the protocol outlined by the German research network for neuropathic pain (DFNS, Rolke et al., 2006). The same researcher (J.W.) conducted the QST assessments.