-
Jan 17, 2025 | 
link.springer.com | Jilin Agricultural
AbstractAmmonia nitrogen (NH3-N) pollution poses a critical challenge to global aquaculture sustainability. Here, we report the isolation and characterization of Bacillus subtilis DM115, a novel probiotic strain that demonstrates exceptional NH3-N degradation capabilities in ornamental fish aquaculture.
-
Oct 10, 2024 | 
veterinaryresearch.biomedcentral.com | Jilin Agricultural
Aeromicrobium camelliae was obtained from the China Microbial Strain Collection and Management Centre, catalogued under strain number JCM 30952, and cultured aerobically at 37 °C for 24 h in a Lysogeny Broth (LB) liquid medium. J774A.1 and MDCK cells are maintained in our laboratory. Our laboratory also preserves the H9N2 strain [49]. The virus’s titre was determined using the half-maximal infective dose (H9N2106.5EID50) in chicken embryos.
-
Jun 27, 2024 | 
link.springer.com | Jilin Agricultural
AbstractChicken coccidiosis is a protozoan disease that leads to considerable economic losses in the poultry industry. Live oocyst vaccination is currently the most effective measure for the prevention of coccidiosis. However, it provides limited protection with several drawbacks, such as poor immunological protection and potential reversion to virulence. Therefore, the development of effective and safe vaccines against chicken coccidiosis is still urgently needed.
-
Jun 27, 2024 | 
parasitesandvectors.biomedcentral.com | Jilin Agricultural
The E. tenella strain was maintained at 4 °C in a 2.5% potassium dichromate solution in our laboratory and passed at least three times a year by chickens. The details of the strains, plasmids and primers used in this study are presented in Table 1. Lactobacillus plantarum strain NC8 was grown in MRS medium without agitation at 30 °C, and Escherichia coli strain DH5α was grown in LB medium. A codon-optimized RON2-Dcpep gene was optimized and synthesized by Sangon Biotech Co., Ltd.
-
Jun 11, 2024 | 
bmcgenomics.biomedcentral.com | Jilin Agricultural
The test animals were selected from Guofeng Livestock Breeding Sheep Farm, China. 20 ewes of similar age and weight were selected and kept in the same pen, and the simultaneous estrus technique was used to ensure that the selected ewes were lambed at the same time. Fifteen lambs were selected and each sheep was numbered.
-
May 26, 2024 | 
bmcgenomics.biomedcentral.com | Jilin Agricultural
This study’s genome sequences, genome annotation files, and amino acid sequences were obtained from the Phytozome 13 plant genome database (https://phytozome-next.jgi.doe.gov/). To identify members of the GmftsH gene family, a combination of methods was employed. Initially, the Arabidopsis thaliana ftsH family protein sequence was acquired from the online TAIR database (https://www.arabidopsis.org/) and subjected to BLASTp alignment.
-
May 23, 2024 | 
bmcgenomics.biomedcentral.com | Jilin Agricultural
Genomic DNA was extracted from leaves of the “Jihong01” safflower variety and sequenced on BGI-SEQ500 and Oxford Nanopore Technologies (ONT) platforms. We obtained 101 Gb short reads and 130 Gb long reads data in total. By a 17-mer frequency statistics, the size of safflower genome was estimated to be 1,061.1 Mb (Figure S1).
-
May 18, 2024 | 
link.springer.com | Jilin Agricultural
AbstractUsing polyaspartic acid (PAsp) and bentonite (BT) as the main raw materials, a new type of degradable soil water retaining agent (PAsp-AA/BT) was synthesized by microwave radiation. The optimum synthesis conditions and comprehensive properties of PAsp-AA/BT were discussed and the structure and surface characteristics of PAspsp-AA/BT were characterized by FTIR, SEM, XRD and TGA in the paper.
-
May 6, 2024 | 
bmcvetres.biomedcentral.com | Jilin Agricultural
BVDV, BRSV, and BPIV-3 were preserved at the Economic Animal Infectious Disease Laboratory of Jilin Agricultural University and were tested by the RT‒qPCR method [38]. Eighty-six cow nasal swabs were collected randomly from farms in Jilin province, some of the cows exhibit symptoms of increased secretion from the eyes and nose.
-
Apr 2, 2024 | 
academic.oup.com | Jilin Agricultural
Ethyl hexanoate, one of the key flavor compounds in Strong-flavor Baijiu. To improve the content of ethyl hexanoate in Strong-flavor Baijiu, a functional strain with high yield of ethyl hexanoate was screened and its ester-producing performance was studied. Upon identification, the strain was classified as Candida sp. and designated as ZY002. Under optimal fermentation conditions, the content of ethyl hexanoate synthesized by ZY002 can be as high as 170.56 mg·L−1.
