Jinhong Xu

May 13, 2024 | cell.com | Weiwei Xue |Huijuan Li |Jinhong Xu |Xiao Yu

Highlights•We establish a cryopreservation method (MEDY) for brain organoids and tissues•MEDY maintains the structure and functional activity of cortical organoids•MEDY can be applied to diverse brain organoids, including patient-derived organoidsMotivationBrain organoids are useful models for modeling brain diseases. However, long-term culture, high costs, and storage problems significantly limit their application in biomedical studies.

Apr 20, 2024 | biorxiv.org | Hui-Juan Li |Xiao Yu |Xi Liu |Jinhong Xu

AbstractSchizophrenia is a highly heritable neurodevelopmental disorder with unknown genetic pathogenic mechanisms. Here, we selected 11 schizophrenia risk genes and generated single-gene-knockout-precise-dorsal/ventral-forebrain-organoids (SKOPOS) via CRISPR-Cas9 system. 90 bulk and 249,430 single-cell RNA-sequencing of SKOPOS revealed that knockout of 11 risk genes lead to different levels of deficits in dorsal/ventral forebrain organoids.