Jinxin Li

1 month ago | nature.com | Wenchang Qian |Penglei Jiang |Mingming Niu |Yujuan Fu |Deyu Huang |Dong Zhang | +12 more

DNA methylation is a significant component in proximal chromatin regulation and plays crucial roles in regulating gene expression and maintaining the repressive state of retrotransposon elements. However, accurate profiling of the proteomics which simultaneously identifies specific DNA sequences and their associated epigenetic modifications remains a challenge. Here, we report a strategy termed SelectID (selective profiling of epigenetic control at genome targets identified by dCas9), which introduces methylated DNA binding domain into dCas9-mediated proximity labeling system to enable in situ protein capture at repetitive elements with 5-methylcytosine (5mC) modifications. SelectID is demonstrated as feasible as dCas9-TurboID system at specific DNA methylation regions, such as the chromosome 9 satellite. Using SelectID, we successfully identify CHD4 as potential repressors of methylated long interspersed nuclear element-1 (LINE-1) retrotransposon through direct binding at the 5’ untranslated region (5’UTR) of young LINE-1 elements. Overall, our SelectID approach has opened up avenues for uncovering potential regulators of specific DNA regions with DNA methylation, which will greatly facilitate future studies on epigenetic regulation. The authors introduce SelectID, an approach combining CRISPR-guided targeting with methylation-sensitive labeling to identify proteins interacting with methylated repetitive sequences. They suggest that CHD4 directly binds young LINE-1 retrotransposons, suppressing their activity.

Dec 11, 2024 | mdpi.com | Tao Chen |Xiaoqi Guo |Jinxin Li

1. IntroductionIn electronic support measurement (ESM) systems, pulse deinterleaving is an important step in intercepting and analyzing radar signals from non-cooperating parties in order to provide basic support [1,2].

Nov 14, 2023 | mdpi.com | Hu Fan |Yan Zhuang |Jinxin Li |Zhi Chen

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Nov 4, 2023 | pubs.rsc.org | Jinxin Li |Hao Zhang |Ling Zhu |Gangcheng Wu

Influence of in vitro gastrointestinal digestion and colonic fermentation on carbonyls scavenging capacity of fiber-bound polyphenols from quinoa Whole grain insoluble dietary fiber (IDF) is a good source of bound-form polyphenols. In the present study, insoluble dietary fiber rich in bound polyphenols (BP-IDF) from quinoa, rye and wheat were prepared.