Jinying Wang

1 month ago | cell.com | Elena Eubanks |Jiya Mody |NEHA PATEL |Benjamin N. Sacks |Mahsa Farahani |Jinying Wang | +24 more

Tissue culture An M17D neuroblastoma cell line stably expressing 3K mutant αSyn tagged with Venus YFP under doxycycline induction was used.18 Those cells were maintained in medium containing Dulbecco’s Modified Eagle Medium (DMEM) with phenol red (#11965118, Thermofisher) plus 10% Fetal Bovine Serum (FBS) (#16000044, Thermofisher), 1% glutamax (#35050061, Thermofisher) and 1% Penicillin-Streptomycin ((10,000 U/mL stock), #15140122, Thermofisher) at 37°C, 5% CO2, and >90% humidity and were...