Jiudi Sun

Oct 16, 2024 | pubs.rsc.org | Shuang G. Zhao |Qiuting Zhang |Ran Luo |Jiudi Sun

Amplification-free miRNA detection with CRISPR/Cas12a System based on Fragment Complementary Activation Strategy CRISPR/Cas12a systems have been repurposed as powerful tools for developing next-generation molecular diagnostics due to their trans-cleavage ability. However, it was long considered that the CRISPR/Cas12a system could only recognize DNA targets.