Junnan Tang

Jan 4, 2024 | nature.com | Yuan Yang |Qiang Huang |Junnan Tang |Wei Zheng

Correction to: Nature Chemical Biology https://doi.org/10.1038/s41589-023-01481-5, published online 5 December 2023. In the version of this article initially published, the affiliations listed for Tao Qi did not include the Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. The error has been corrected in the HTML and PDF versions of the article. About this articleQi, T., Wang, Y., Yang, Y. et al.

Dec 4, 2023 | nature.com | Yuan Yang |Qiang Huang |Junnan Tang |Wei Zheng

AbstractCompact Cas9 nucleases hold great promise for therapeutic applications. Although several compact Cas9 nucleases have been developed, many genomic loci still could not be edited due to a lack of protospacer adjacent motifs (PAMs). We previously developed a compact SlugCas9 recognizing an NNGG PAM. Here we demonstrate that SlugCas9 displays comparable activity to SpCas9. We developed a simple phage-assisted evolution to engineer SlugCas9 for unique PAM requirements.