Karin Schnetz

Feb 22, 2024 | nature.com | Sabrina Schulze |Gottfried J. Palm |Susanne Sievers |Ulrich Baumann |Karin Schnetz

AbstractThe Escherichia coli TetR-related transcriptional regulator RutR is involved in the coordination of pyrimidine and purine metabolism. Here we report that lysine acetylation modulates RutR function. Applying the genetic code expansion concept, we produced site-specifically lysine-acetylated RutR proteins. The crystal structure of lysine-acetylated RutR reveals how acetylation switches off RutR-DNA-binding. We apply the genetic code expansion concept in E.