Kshitiza Mohan Dhyani

Dec 4, 2024 | elifesciences.org | Sugith Badugu |Kshitiza Mohan Dhyani |Manoj Thakur |Kalappa Muniyappa

eLife Assessment This manuscript reports important data providing evidence that a 42 amino acid region of Rev7 is necessary and sufficient for interaction with the Rad50-Mre11-Xrs2 complex in budding yeast. The authors conclude that Rev7 inhibits the Rad50 ATPase and the Mre11 nuclease with the exception of ssDNA exonuclease activity.

Sep 26, 2024 | biorxiv.org | Sugith Badugu |Kshitiza Mohan Dhyani |Manoj Thakur |Kalappa Muniyappa

AbstractRecent studies have shown that, in human cancer cells, the tetrameric Shieldin complex (comprising REV7, SHLD1, SHLD2, and SHLD3) facilitates non-homologous end-joining (NHEJ) while blocking homologous recombination (HR). Surprisingly, several eukaryotic species lack SHLD1, SHLD2 and SHLD3 orthologs, suggesting that Rev7 may leverage an alternative mechanism to regulate the double-strand break (DSB) repair pathway choice.

Feb 21, 2024 | biorxiv.org | Sugith Badugu |Manoj Thakur |Kalappa Muniyappa |Kshitiza Mohan Dhyani

AbstractRecent studies in cancer cell lines have shown that the tetrameric Shieldin complex (comprising REV7, SHLD1, SHLD2, and SHLD3) facilitates non-homologous end-joining (NHEJ), while blocking homologous recombination (HR). Surprisingly, several eukaryotic species lack SHLD1, SHLD2 and SHLD3 orthologs, suggesting that Rev7 may leverage an alternative mechanism to regulate the double-strand break (DSB) repair pathway choice.