Kyle Barrie

Oct 24, 2024 | nature.com | Kyle Barrie |Grzegorz Rebowski |Roberto Dominguez

AbstractGelsolin is the prototypical member of a family of Ca2+-activated F-actin severing and capping proteins. Here we report structures of Ca2+-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin’s six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3—a fragment observed during apoptosis—binding on both sides of F-actin.

Sep 10, 2024 | biorxiv.org | Kyle Barrie |Grzegorz Rebowski |Roberto Dominguez

AbstractGelsolin is the prototypical member of a family of Ca2+-dependent F-actin severing and capping proteins. A structure of Ca2+-bound full-length gelsolin at the barbed end shows domains G1G6 and the inter-domain linkers wrapping around F-actin. Another structure shows domains G1G3, a fragment produced during apoptosis, on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3.

Jun 6, 2024 | nature.com | Kyle Barrie |Roberto Dominguez

AbstractHumans express fifteen formins, playing crucial roles in actin-based processes, such as cytokinesis, cell motility, and mechanotransduction 1,2. However, the lack of structures bound to the actin filament (F-actin) has been a major impediment to understanding formin function. While formins are known for their ability to nucleate and elongate F-actin 3-7, some formins can additionally depolymerize, sever, or bundle F-actin.

Jul 10, 2023 | mondaq.com | Kyle Barrie

New Brunswick's new Fiduciaries Access to Digital Assets Act received Royal Assent on December 16, 2022. The new Act is the first of its kind in New Brunswick and makes the Province one of the few Canadian jurisdictions to have such a piece of legislation. The purpose of the Act is to provide a framework for individuals to plan what will happen to their digital assets upon death or incapacity.