Kyoung-Ran Kim

May 29, 2024 | pubs.rsc.org | Binh Tran |Bình Trần |Seulgi Go |Kyoung-Ran Kim |Hak Suk Chung

The positional and numerical effect of N6-methyladenosine in tracrRNA on the DNA cleavage activity of Cas9† Post-transcriptional modifications on the guide RNAs utilized in the Cas9 system may have the potential to impact the activity of Cas9. In this study, we synthesized a series of tracrRNAs containing N6-methyadenosine (m6A), a prevalent post-transcriptional modification, at various positions.