Larissa S. Arantes

Jan 7, 2025 | onlinelibrary.wiley.com | Marisol Domínguez |Larissa S. Arantes |Pablo D. Lavinia |Nicole Bergjürgen

1 Introduction The unprecedented population declines in global biodiversity highlight the urgent need to understand, monitor, and develop effective conservation strategies (Ceballos et al. 2015). Conservation genomics emerges as a powerful tool to address these challenges by assessing conservation status, monitoring genetic diversity, delineating conservation units within species, combating illegal wildlife trafficking, and unraveling complex conservation issues such as hybridization.

Sep 27, 2024 | biorxiv.org | Bernhard Bein |Ioannis Chrysostomakis |Larissa S. Arantes |Tom Brown

AbstractMuseum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assembled the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit.

Sep 5, 2024 | onlinelibrary.wiley.com | Jilda Alicia Caccavo |BeGenDiv Berlin Germany |IZW Berlin Germany |Larissa S. Arantes

1 INTRODUCTION Otoliths (fish ear bones) are routinely collected as part of stock assessments in order to determine age and growth parameters (Jones, 1992). Otolith collection protocols typically require the dissection of the otolith pair from the fish head, removal of visible blood and tissue from the otolith surface and final storage in a paper envelope (e.g. CCAMLR, 2023; Chittaro et al., 2020; GFCM, 2018; SEAFO, 2015).

Sep 5, 2024 | onlinelibrary.wiley.com | Jilda Alicia Caccavo |Larissa S. Arantes |BeGenDiv Berlin Germany |IZW Berlin Germany

1 INTRODUCTION Otoliths (fish ear bones) are routinely collected as part of stock assessments in order to determine age and growth parameters (Jones, 1992). Otolith collection protocols typically require the dissection of the otolith pair from the fish head, removal of visible blood and tissue from the otolith surface and final storage in a paper envelope (e.g. CCAMLR, 2023; Chittaro et al., 2020; GFCM, 2018; SEAFO, 2015).

Mar 7, 2024 | biorxiv.org | Ioannis Chrysostomakis |Larissa S. Arantes |Tom Brown |Bernhard Bein

AbstractAdvances in long-read sequencing technology have shifted a key challenge in biodiversity genomics from DNA sequencing and genome assembly to obtaining suitable samples. Although flash-frozen samples remain the gold standard, they are often unattainable. In contrast, natural history collections worldwide harbor millions of ethanol-preserved samples, but such samples remain largely unutilized for long-read sequencing.