Manikandan Ramasamy

May 22, 2024 | onlinelibrary.wiley.com | Manikandan Ramasamy |Renesh H Bedre |Sonia Irigoyen |Mohan Singh Rajkumar

Candidatus Liberibacter solanacearum (CLso) is a phloem-limited, fastidious bacterium associated with the potato (Solanum tuberosum) zebra chip disease. It is transmitted by the potato psyllid (Bactericera cockerelli Šulc.) and causes significant economic losses globally (Mora et al., 2021). Developing disease resistance by conventional breeding has shown limited success (Mora et al., 2022), thus necessitating new genetic engineering or genome editing approaches.

Jun 14, 2023 | onlinelibrary.wiley.com | Michelle Dominguez |Carmen Padilla |Manikandan Ramasamy |Sonia Irigoyen

Plant transformation is widely used in functional genetics and bioengineering (BE)-based crop improvement. With the advent and deployment of CRISPR-based genome editing technology and increased public awareness of the safety of BE crops, the applications of plant genetic engineering are bound to increase. Multiple methods have been used for plant transformation, such as DNA particle bombardment, PEG-mediated transfection, and Agrobacterium-mediated transformation.