Marta Medina-Carbonero

Oct 3, 2024 | onlinelibrary.wiley.com | Zichen Wang |Hiroyuki Hakozaki |Gillian McMahon |Marta Medina-Carbonero

1 INTRODUCTION Light-sheet fluorescence microscopy (LSFM) is a principal type of fluorescence microscopy extensively employed in cell and developmental biology.1-10 By avoiding unnecessary photobleaching above and below the in-focus plane (compared with widefield and confocal microscopes), while rejecting out-of-focus background (compared with widefield microscope), LSFM offers significantly enhanced temporal resolution and duration for three-dimensional time-lapse imaging (4D; x, y, z,...

May 30, 2024 | biorxiv.org | Zichen Wang |Hiroyuki Hakozaki |Gillian McMahon |Marta Medina-Carbonero

AbstractLight-sheet fluorescence microscopy (LSFM), a prominent fluorescence microscopy technique, offers enhanced temporal resolution for imaging biological samples in four dimensions (4D; x, y, z, time). Some of the most recent implementations, including inverted selective plane illumination microscopy (iSPIM) and lattice light-sheet microscopy (LLSM), rely on a tilting of the sample plane with respect to the light sheet of 30-45 degrees to ease sample preparation.