Mattias Winsen

Dec 5, 2024 | biorxiv.org | Jacob Bradford |Divya Joy |Mattias Winsen |Nicholas Meurant

AbstractGene editing has been revolutionised by the CRISPR-Cas9 technology. The versatility and ease-of-use of the technology far exceeds its predecessors, however, the selection of a high-quality guide RNA (gRNA) is critical to directing it to a target site. Selecting gRNA calls upon high-performance algorithms that evaluate nuclease activity at on-target and off-target sites.