Max C. Gamill

2 months ago | biorxiv.org | Elizabeth Holmes |Max C. Gamill |James Provan |Laura Wiggins

AbstractThe topology of DNA plays a crucial role in the regulation of cellular processes and genome stability. Despite its significance, DNA topology remains challenging to determine due to the length and conformational complexity of individual topologically constrained DNA molecules. We demonstrate unparalleled resolution of complex DNA topologies using Atomic Force Microscopy (AFM) in aqueous conditions.

Sep 9, 2024 | biorxiv.org | Elizabeth Holmes |Max C. Gamill |James Provan |Laura Wiggins

AbstractThe topology of DNA plays a crucial role in the regulation of cellular processes and genome stability. Despite its significance, DNA topology is challenging to explicitly determine due to the length and conformational complexity of individual topologically constrained DNA molecules. We demonstrate unparalleled resolution of complex DNA topologies in aqueous solutions, achieving resolution of the double helix around two intertwined molecules using atomic force microscopy (AFM).

Jun 28, 2024 | biorxiv.org | Elizabeth Holmes |James Provan |Laura Wiggins |Max C. Gamill

AbstractThe intricate topology of DNA plays a crucial role in the regulation of cellular processes and genome stability. Despite its significance, DNA topology is challenging to explicitly determine due to the length and conformational complexity of individual topologically constrained DNA molecules. Here, we introduce an innovative approach combining high-resolution Atomic Force Microscopy (AFM) imaging with automated computational analysis to directly determine DNA topology.