Michal Kabza

Dec 1, 2023 | biorxiv.org | Ashley Byrne |Alexander Ritter |Kostianna Sereti |Michal Kabza

AbstractAccurate detection and quantification of mRNA isoforms from nanopore long-read sequencing remains challenged by technical noise, particularly in single cells. To address this, we introduce Isosceles, a computational toolkit that outperforms other methods in isoform detection sensitivity and quantification accuracy across single-cell, pseudo-bulk and bulk resolution levels, as demonstrated using synthetic and biologically-derived datasets.