Nicholas T. Ingolia

2 months ago | elifesciences.org | Santi Mestre-Fos |Lucas Ferguson |Marena Trinidad |Nicholas T. Ingolia

Ribosome profiling computational analysis Request a detailed protocol Ribosome profiling and Total RNA cDNA libraries were sequenced on an Illumina NovaSeq 150PE platform.

Aug 31, 2024 | biorxiv.org | Santi Mestre-Fos |Lucas Ferguson |Marena Trinidad |Nicholas T. Ingolia

AbstractStem cell differentiation involves a global increase in protein synthesis to meet the demands of specialized cell types. However, the molecular mechanisms underlying this translational burst and the involvement of initiation factors remains largely unknown. Here, we investigate the role of eukaryotic initiation factor 3 (eIF3) in early differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (NPCs).

Nov 11, 2023 | biorxiv.org | Santi Mestre-Fos |Lucas Ferguson |Marena Trinidad |Nicholas T. Ingolia

AbstractStem cell differentiation involves a global increase in protein synthesis to meet the demands of specialized cell types. However, the molecular mechanisms underlying this translational burst and the involvement of initiation factors remains largely unknown. Here, we investigate the roles of eukaryotic initiation factor 3 (eIF3) in early differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (NPCs).

May 25, 2023 | nature.com | Nicholas T. Ingolia

Gebauer, F., Preiss, T. & Hentze, M. W. From cis-regulatory elements to complex RNPs and back. Cold Spring Harb. Perspect. Biol. 4, a012245 (2012). Article  PubMed  PubMed Central  Google Scholar  Hafner, M. et al. Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. Cell 141, 129–141 (2010). Article  CAS  PubMed  PubMed Central  Google Scholar  Gerstberger, S., Hafner, M. & Tuschl, T. A census of human RNA-binding proteins. Nat. Rev. Genet. 15, 829–845 (2014).