Noor Al-Sayyad

Oct 2, 2024 | biorxiv.org | Honglue Shi |KEVIN WASKO |Marena Trinidad |Noor Al-Sayyad

AbstractRNA-guided CRISPR-Cas enzymes initiate programmable genome editing by recognizing a 20-base-pair DNA sequence adjacent to a short protospacer-adjacent motif (PAM). To uncover the molecular determinants of high-efficiency editing, we conducted biochemical, biophysical and cell-based assays on S. pyogenes Cas9 (SpyCas9) variants with wide-ranging genome editing efficiencies that differ in PAM binding specificity.