Paulina N Smaruj

Aug 4, 2024 | biorxiv.org | David Kelley |Geoffrey Fudenberg |Paulina N Smaruj |Fahad Kamulegeya

AbstractInterphase mammalian genomes are folded in 3D with complex locus-specific patterns that impact gene regulation. CTCF (CCCTC-binding factor) is a key architectural protein that binds specific DNA sites, halts cohesin-mediated loop extrusion, and enables long-range chromatin interactions. There are hundreds of thousands of annotated CTCF-binding sites in mammalian genomes; disruptions of some result in distinct phenotypes, while others have no visible effect.