Philipp Hoffmeister

Jul 10, 2024 | biorxiv.org | Duyen Huynh |Philipp Hoffmeister |Tobias Friedrich |Kefan Zhang

AbstractTranscription factors (TFs) such as the central DNA-binding hub in Notch signal transduction, RBPJ, bind to specific DNA sequences to regulate gene transcription. How the efficiency of gene regulation depends on the TF-DNA binding kinetics and cofactor interactions is mostly unknown.

Dec 20, 2023 | biorxiv.org | Tobias Friedrich |Kefan Zhang |Duyen Huynh |Philipp Hoffmeister

AbstractTranscription factors such as RBPJ in Notch signal transduction bind to specific DNA sequences and initiate either repression or activation of genes. Which sites they select and how often and long they bind affects the efficiency of gene regulation. To resolve the underpinnings of RBPJ-DNA binding, we determined the in vivo binding free energy landscape of RBPJ using live-cell single-molecule tracking and genome-wide chromatin immunoprecipitation.