Rea L. Antoniou-Kourounioti

Nov 14, 2023 | nature.com | Jianping Zhang |Shisheng Chen |Burkhard Steuernagel |Tim Hewitt |Hongna Li |Rea L. Antoniou-Kourounioti | +5 more

AbstractMost rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h.

Feb 16, 2023 | nature.com | Rea L. Antoniou-Kourounioti |Burkhard Steuernagel |Luzie U. Wingen |Erin Baggs |Jonathan Clarke |Sanu Arora | +2 more

Watkins panel configurationUsing the SSR genotype data from Wingen et al. (2014) (ref. 27), a core set of 300 genetically diverse wheat landraces with spring growth habit were selected from the Watkins collection (Supplementary Fig. 1 and Supplementary Table 2) along with 20 non-Watkins lines. The DNA was extracted following a modified CTAB protocol28.