Riley N Quijano

Sep 30, 2024 | nature.com | Victoria A. Maola |Mohammad Hajjar |Joy Lee |Manuel Holguin |Riley N Quijano |Katherine Ho | +3 more

AbstractReprogramming DNA polymerases to synthesize xeno-nucleic acids (XNAs) is an important challenge that tests current enzyme engineering tools. Here we describe an evolutionary campaign aimed at generating an XNA polymerase that can efficiently make α-l-threofuranosyl nucleic acid (TNA)—an artificial genetic polymer that is recalcitrant to nucleases and resistant to acid-mediated degradation.