Ronnie P-A. Berntsson

Feb 12, 2024 | nature.com | Laura Alvarez |Gabriel Torrens |Josy ter Beek |Vega Miguel-Ruano |Federico Gago |Ronnie P-A. Berntsson

AbstractThe bacterial cell-wall peptidoglycan is made of glycan strands crosslinked by short peptide stems. Crosslinks are catalyzed by DD-transpeptidases (4,3-crosslinks) and LD-transpeptidases (3,3-crosslinks). However, recent research on non-model species has revealed novel crosslink types, suggesting the existence of uncharacterized enzymes. Here, we identify an LD-transpeptidase, LDTGo, that generates 1,3-crosslinks in the acetic-acid bacterium Gluconobacter oxydans.