Sarah V. Faull

Jan 8, 2025 | nature.com | Matthew Peach |Sarah V. Faull

AbstractHuman DNA licensing initiates replication fork assembly and DNA replication. This reaction promotes the loading of the hMCM2-7 complex on DNA, which represents the core of the replicative helicase that unwinds DNA during S-phase. Here, we report the reconstitution of human DNA licensing using purified proteins. We showed that the in vitro reaction is specific and results in the assembly of high-salt resistant hMCM2-7 double-hexamers.

Mar 27, 2024 | lexology.com | Louisa Chambers |James Longster |Dan Reavill |Helen Reddish |Ben Chivers |Jonathan Rush | +40 more

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