Satoshi Hasegawa

Oct 11, 2024 | biorxiv.org | Yuteng Wang |Pablo Vergara |Satoshi Hasegawa |Naoki Tomita

AbstractA miniaturized microscope (i.e., miniscope) enables the imaging of neuronal activity using calcium sensors while simultaneously manipulating that activity using opsins in freely moving animals. However, many miniscopes use light-emitting diodes with broadband emission, leading to unintentional opsin stimulation by light intended solely for calcium sensor activation (a phenomenon referred to as "biological crosstalk").